Effects of sodium butyrate on proliferation and differentiation of human pancreatic cancer cell line ASPC-1
10.3760/cma.j.issn.1007-8118.2011.01.014
- VernacularTitle:丁酸钠对人胰腺癌细胞ASPC-1生长的影响及其机制的研究
- Author:
Wei ZHOU
;
Li WANG
;
Tao LIU
;
Tongling WANG
;
Chunyou WANG
- Publication Type:Journal Article
- Keywords:
Pancreatic cancer;
Sodium butyrate
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(1):46-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sodium butyrate(NaBT) on proliferation of human pancreatic cancer cell line ASPC-1 and explore the possible mechanism. Methods The methylthiazolyl tetrazolium assay (MTT) method was used to detect cell proliferation and draw a curve. The cell apoptosis and cell cycle were determined with flow cytometry. Western blot was used to study the effect NaBT on the pancreatic cancer cells and explore its mechansim. Real-time PCR was employed to assess the expression levels of p53, p21, bcl-2 and cell cycle regulation gene p21. Results After incubation with different concentrations of NaBT for 24 to 72 h, ASPC-1 cell proliferation was inhibited dramatically. NaBT induced an increase of G0/G1 phase cells and a significant decrease in the ratio of S phase cells. The expression of p21 and bax was up-regulated at protein and mRNA level. The expression of bcl-2 was down-regulated at protein and mRNA level. There was no significant difference in the expression of p53 at protein and mRNA level. Conclusion TSA-induced growth inhibition is associated with a block in the G0/G1 phase and apoptosis, which may occur through down-regulating the expression of apoptosis gene bcl-2 and up-regulating the expression of cell cycle regulation gene p21and pro-apoptotic gene bax.
