Effect of bone mesenchymal stem cells on toll-like receptor 2/4 expression in lungs of rats with acute hemorrhagic necrotizing pancreatitis
10.3760/cma.j.issn.1007-8118.2011.01.013
- VernacularTitle:骨髓间充质干细胞对急性出血坏死性胰腺炎大鼠肺组织Toll样受体2/4mRNA表达的影响及意义
- Author:
Dequan LI
;
Lin WANG
;
Heshui WU
;
Hailong WU
;
Jinghui ZHANG
;
Yuan TIAN
- Publication Type:Journal Article
- Keywords:
Toll-like receptor;
Acute hemorrhagic necrotizing pancreatitis;
Lung;
BMSCs
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(1):42-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of bone mesenchymal stem cells on Toll-like receptors (TLR) 2/4 expression in the lungs of rats with acute hemorrhagic necrotizing pancreatitis (AHNP). Methods Seventy SD male rats were randomly divided into sham-operation group (n=10), AHNP group(n=30) and MSCs-treated group(n=30). Masc rate of BMSCs with surface mark were measured by flow cytometer. TLR2/4mRNA expression in the the lung were measured by RT-PCR, and The ratio of Wet/dry and lung histological changs were observed. Results TLR2/4 mRNA could be detected in the lungs with low values in sham-operation group, markedly increased in 3 h, and peaked in 12 h in AHNP group (P＜0.05). Lung injuries were aggravated and the levels of TNF-α in the lung were increased (P＜0. 05) . Treatment with MSCs could effectively inhibit TLR2/4 mRNA expression and relieve lung injuries. The levels of TNF-α in the lung were decreased (P＜0.05). Conclusions The expression of TLR2/4 mRNA is increased in the lungs in AHNP and the lung injuries are aggravated. MSCs could markedly inhibit TLR2/4 mRNA expression in the lungs in AHNP, which would lead to relief of lung injury.
