Effects of IL-6 on invasion of pancreatic cancer cells and its mechanism
10.3760/cma.j.issn.1007-8118.2011.01.015
- VernacularTitle:白细胞介素6促进人胰腺癌细胞的侵袭及机制探讨
- Author:
Guang YANG
;
Zhengjun QIU
;
Jun LIU
;
Weimin BI
;
Gang CUI
;
Chen HUANG
- Publication Type:Journal Article
- Keywords:
Pancreatic cancer;
Interleukin-6;
STAT3;
Invasion
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(1):50-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanism of IL-6 on invasion and metastasis of human pancreatic cancer cells. Methods IL-6 was added into the culture media of human pancreatic cancer cells Capan-2 and SW1990. Cell growth was measured by MTT assay. Western blot and immunocytochemistry were performed to detect Phosphorylated STAT3 (P-STAT3) protein. VEGF and MMP-2 mRNA and protein expression were examined using fluorescence quantitative polymerase chain reaction (RT-PCR) and Western blot, respectively. The invasion ability of SW1990 and Capan2 cells was determined by cell invasion assay in vitro. Results 100 ng/mL IL-6 significantly promoted growth and invasion ability of Capan-2 and SW1990 cells (P＜0.05). The use of IL-6 not only markedly increased the protein expression of P-STAT3, VEGF and MMP-2, but also greatly increased the mRNA expression of MMP-2 and VEGF. Conclusions STAT3 signal transducer pathway activation with IL-6 can promote the invasion ability of pancreatic cancer cells in vitro through up-regulation of MMP-2 and VEGF expression. STAT3 signal transducer may provide a novel therapeutic target for the treatment of pancreatic cancer.
