Determination of emodin in Dabaidu capsule by HPLC
10.3760/cma.j.issn.1673-4246.2011.01.014
- VernacularTitle:高效液相色谱法测定大败毒胶囊中大黄素含量的研究
- Author:
Xintao FAN
- Publication Type:Journal Article
- Keywords:
Emodin;
HPLC;
Determination
- From:
International Journal of Traditional Chinese Medicine
2011;33(1):35-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of emodin in Dabaidu capsule. Methods The chromatographic system was adopted C18 column, using methanol -0.1% phosphoric acid (70∶30) as mobile phase. The content was detected at a waveleng of 254 nm and the flow rate was 1.0 ml/min.Results The emodin have a good tincar rolatim in the range of 6.181 ～ 18.54 μg/ml (r=0.9999), the average recovery rate was 98.93% and RSD = 0.65% (n = 6) . Conclusion The established method is accuracy,sensitive and simple.
