Effects of remifentanil on lipid peroxidation during hemorrhagic shock-induced acute lung injury in rabbits
10.3760/cma.j.issn.0254-1416.2010.11.028
- VernacularTitle:瑞芬太尼对失血性休克兔急性肺损伤时脂质过氧化反应的影响
- Author:
Yaru CHEN
;
Jinhai MENG
;
Zhenzhou LI
;
Xinli NI
;
Xuexin CHEN
;
Hanxiang MA
;
Fuqiang MA
- Publication Type:Journal Article
- Keywords:
Piperidines;
Respiratory distress syndrome,adult;
Shock,hemorrhagic;
Lipid peroxidation
- From:
Chinese Journal of Anesthesiology
2010;30(11):1375-1377
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of remifentanil on lipid peroxidation druing hemorrhagic shock-induced acute lung injury (ALI) in rabbits. Methods Thirty-two healthy adult rabbits weighing 2.0-2.5 kg were randomly divided into 4 groups (n = 8 each): sham operation group (group S); ALI group; low-dose remifentanil group (group LR); high-dose remifentanil group (group HR). The left femoral artery was cannulated for blood-letting and blood sampling. The right femoral artery was cannulated for remifentanil administration. The model of hemorrhagic shock was established by modified Wigger' s methods. In group S, only cannulation was performed. In group LR and HR, remifentanil was infused intraperitoneally at 0.66 and 1.32 μg·kg-1 ·min-1 15 min before blood-letting respectively, while group ALI received equal volume of normal saline instead. Arterial blood samples were taken at 0, 20,70 and 100 min after blood-letting (T1-4) for blood gas analysis. The animals were then sacrificed and the lungs were immediately removed for histological examination with light microscope and determination of W/D lung weight ratio, MDA content and SOD activity. Results W/D ratio and MDA content were significantly increased, while SOD activity was significantly decreased in group ALI compared with group S (P ＜0.05). The pH value at T2 and PaO2 at T2-4 in group LR and the pH value and PaCO2 at T2-4 in group HR were significantly higher than those in group ALI (P ＜ 0.05). W/D ratio and MDA content were significantly lower,while SOD activity was significantly higher in group LR and HR than in group ALI, and in group HR than in group LR (P ＜ 0.05). Remifentanil infusion significantly attenuated the pathologic changes in a dose-dependent manner. Conclusion Remifentanil pretreatment can attenuate hemorrhagic shock-induced ALI through inhibiting lipid peroxidation in rabbits.
