Effects of insulin and β-adrenergic agonist on adiponectin secretion and expression in rats
10.3760/cma.j.issn.1000-6699.2011.01.018
- VernacularTitle:胰岛素和β肾上腺素受体激动剂对大鼠脂联素分泌和表达的影响
- Author:
Gang LI
;
Li CONG
;
Na YIN
;
Qiang LI
;
Wei WANG
;
Jinchao ZHANG
- Publication Type:Journal Article
- Keywords:
Adiponectin;
Insulin;
β-adrenergic agonists;
Insulin clamp
- From:
Chinese Journal of Endocrinology and Metabolism
2011;27(1):62-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the potential mechanisms of regulating adiponectin secretion and expression in vivo in rats.Methods To observe the regulation of adiponectin by fasing-refeeding and β-adrenergic agonists, male Wistar rats were fasted for 18 h and allowed to refeed or a β3-adrenergic receptor agonist was infused into refeeding rats.The effects of insulin clamp on adiponectin secretion and expression, including euglycemichyperinsulinemic clamp and hyperglycemic-hyperinsulinemic clamp, were also investigated.Plasma adiponectin level was determined by radioimmunoassay.Adiponectin mRNA expression in adipose tissue of rats was detected by realtime PCR.Results (1) Refeeding 18 h fasted rats increased plasma adiponectin concentration (about 2-fold) and adipose tissue adiponectin expression (about 3-fold), which were completely blocked by administration of β-adrenergic agonist.(2) Hyperinsulinemic clamp increased plasma adiponectin concentration and adiponectin gene expression in adipose tissue.Conclusions Adiponectin secretion and expression are acutely regulated in vivo by nutritional status.Insulin and β-adrenergic agonists regulate adiponectin secretion and expression in adipose tissue.
