Effects of propofol on mitochondrial membrane permeability during hypoxia/reoxygenation in rat hippocampal neurons
10.3760/cma.j.issn.0254-1416.2010.11.030
- VernacularTitle:异丙酚对大鼠海马神经元缺氧复氧时线粒体膜通透性的影响
- Author:
Na ZHANG
;
Lingzhi YU
;
Ke ZHANG
- Publication Type:Journal Article
- Keywords:
Propofol;
Cell hypoxia;
Oxygen;
Mitochondrial membranes;
Permeability;
Hippocampus;
Neurons
- From:
Chinese Journal of Anesthesiology
2010;30(11):1381-1383
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of propofol on mitochondrial membrane permeability during hypoxia/reoxygenation (H/R) in rat hippocampal neurons. Methods Primary cultured hippocampal neurons of fetal rats obtained from Wistar (17-18 days of gestation) were randomly divided into 3 groups: Ⅰ control group (group C), Ⅱ H/R group and Ⅲ propofol + H/R group. Neurons were cultured in the culture medium with combined oxygen glucose deprivation for 2 h followed by reoxygenation in group Ⅱ and Ⅲ. In group Ⅲ propofol was added to the culture medium with the final concentration of 20 μ mol/L before combined oxygen glucose deprivation.Neuronal viability was detected by MTT assay and mitochondrial membrane potential (MMP) with flow cytometry at 0, 4, 8, 12 and 24 h after reoxygenation (T1-5) and the permeability of cell membrane and mitochondrial membrane was monitored at T5 using laser confocal scanning microscope. Results The neuronal viability and MMP were significantly decreased (P ＜ 0.05), while the permeability of cell membrane and mitochondrial membrane was increased at T5 in group Ⅱ as compared to group Ⅰ . The neuronal viability at T1-4 and MMP at T1-5 were significantly increased (P ＜ 0.05), while the permeability of cell membrane and mitochondrial membrane was decreased at T5 in group Ⅲ compared to group Ⅱ . Conclusion Propofol can protect rat hippocampal neurons against H/R injury through increasing MMP, improving the cell and mitochondrial membrane permeability, and increasing the neuronal viability.
