Effect of flurbiprofen pretreatment on permeability of blood-brain barrier in a rat model of global cerbral ischemia-reperfusion injury
10.3760/cma.j.issn.0254-1416.2010.11.031
- VernacularTitle:氟比洛芬酯预先给药对全脑缺血再灌注大鼠血脑屏障通透性的影响
- Author:
Huisheng WU
;
Peipei GUO
;
Zongze ZHANG
;
Danyan LIU
- Publication Type:Journal Article
- Keywords:
Flurbiprofen;
Reperfusion injury;
Brain;
Blood-brain barrier
- From:
Chinese Journal of Anesthesiology
2010;30(11):1384-1387
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of flurbiprofen pretreatment on the permeability of bloodbrain barrier in a rat model of global cerbral ischemia-reperfusion (I/R) injury. Methods Forty-five male SD rats weighing 300-350 g were randomly divided into 3 groups (n = 15 each): sham operation group (group S); global cerebral I/R group (group I/R); flurbiprofen 10 mg/kg + global cerebral I/R group (group F). Global cerebral ischemia was induced by 20 min occlusion of bilateral common carotid arteries combined with hypotension (MAP maintained at 35-45 mm Hg). In group F, flurbiprofen 10 mg/kg was injected iv at 15 min before ischemia. Evans blue 3 ml/kg was injectcd iv at 24 h of reperfusion, then the rats were sacrificed and their brains were immediately removed for determination of the apoptosis rate, brain water content, Evans blue content, TNF-α, IL-1β and IL-10 content, and microscopic examination. Results The apoptosis rate, brain water content, Evans blue content, and TNF-α, IL-1β and IL-10 content were significantly higher in group I/R and F than in group S (P ＜ 0.05 or 0.01).The apoptosis rate, brain water content, and Evans blue content and TNF-α and IL-1β content were significantly lower, while IL-10 content was higher in group F than in group I/R (P ＜ 0.01). Global cerbral I/R-induced changes were significantly attenuated in group F. Conclusion Pretreatment with flurbiprofen can protect bloodbrain barrier against cerebral I/R injury by inhibition of the inflammatory reaction.
