Effects of remifentanil on NF-Bκ activity in a rabbit model of myocardial ischemia/reperfusion injury
10.37601cma.j.issn.0254-1416.2010.11.025
- VernacularTitle:瑞芬太尼对兔心肌缺血再灌注时NF-κB活性的影响
- Author:
Wei WANG
;
Xinyuan YANG
;
Xinjing ZHAO
- Publication Type:Journal Article
- Keywords:
Piperidines;
Myocardial reperfusion injury;
NF-kappa B
- From:
Chinese Journal of Anesthesiology
2010;30(11):1364-1366
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of remifentanil on NF-κB activity in a rabbit model of myocardial ischemia/reperfusion (I/R) injury. Methods Fifty New Zealand white rabbits were randomly divided into 5 groups (n = 10 each): sham operation group (group S); group I/R; low dose of remifentanil group (group L); median dose of remifentanil group (group M); high dose of remifentanil group (group H). In group I/R, L,M and H, myocardial I/R was produced by occlusion of left anterior descending artery for 30 min followed by 120 min reperfusion.In group S and I/R,10 min before ischemia,normal saline was infused at 5 ml·l·h-1 via the external jugular vein until the end of 120 min reperfusion. In group L, M and H, remifentanil was infused at 1, 5 and 10ug·kg·min-1 respectively 10 min before ischemia until the end of 120 min reperfusion,and the other procedures were the same as those in group I/R. The myocardial tissues were taken at the end of 120 min reperfusion for determination of NF-κB expression which was used to reflect the activity of NF-κB and microscopic examination. Results The activity of NF-κB was significantly higher in group I/R, L, M and H than in group S. The activity of NF-κB was gradually decreased with the increase in the dose of remifentanil in group L, M and H compared with group I/R. The microscopic examination showed that remifentanil significantly attenuated I/R-induced injury in a dose-dependent manner. Conclusion Infusion of remifentanil reduces myocardial I/R injury through decreasing the activity of NF-κB in a doee-dependent manner.
