Effect of sevoflurane postconditioning on activities of Na+-K+-ATPase and Ca2+ -Mg2+ -ATPase during myocardial ischemia-reperfusion in rats
10.3760/cma.j.issn.0254-1416.2010.10.008
- VernacularTitle:七氟醚后处理对大鼠心肌缺血再灌注时Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性的影响
- Author:
Yue LIU
;
Jin ZHANG
;
Ya LIU
;
Jianjun REN
;
Lining HUANG
;
Xuze LI
;
Zhenming DONG
- Publication Type:Journal Article
- Keywords:
Sodium-potassium-exchanging ATPase;
Ca(2 + ) Mg(2 + )-ATPase;
Myocardial reperfusion injury;
Sevoflurane postconditioning
- From:
Chinese Journal of Anesthesiology
2010;30(10):1179-1181
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sevoflurane postconditioning on the activities of Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase during myocardial ischemia-reperfusion (I/R) in rats and the possible mechanism. Methods Forty-five healthy male Wistar rats weighing 250-280 g were randomly divided into 3 groups ( n = 15 each): sham operation group (group S), I/R group and sevoflurane postconditioning group (group Spo). Myocardial I/R was induced by occlusion of anterior descending branch of left coronary artery for 30 min followed by 120 min of reperfusion. In group S the anterior descending branch was only exposed but not ligated. Group Spo received 5 min inhlation of 2.5% sevoflurane 1 min before reperfusion. The myocardial tissues were taken at 2 h of reperfusion for determination of infarct size and activities of Na+ -K+ -ATPase and Ca2 * -Mg2 * -ATPase. Results The infarct size was significantly larger and the activities of Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase were signifi cantly lower in group I/R than in group S ( P ＜ 0.05). The infarct size was significantly smaller and the activities of Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase were significantly higher in group Spo than in group I/R (P ＜ 0.05 ). Conclusion Sevoflurane postconditioning can reduce myocardial I/R injury through increasing the activities of Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase.
