Promotion of calcium oxalate crystallization after human renal tubular epithelial cell injury

VernacularTitle:近端肾小管上皮细胞损伤后对草酸钙结晶的促进作用
Author: Xiuqiong YAO ; Rue YANG ; Suiping DENG ; Li KUANG ; Fengxin WANG ; Jianming OUYANG ; Zexuan SU
Publication Type:Journal Article
Keywords: Calcium oxalate; Crystallization; Biomineralization; Cell injury; Kidney calculi
From: Chinese Journal of Urology 2011;32(1):7-10
CountryChina
Language:Chinese
Abstract: Objective To investigate the injury caused by hydrogen peroxide (H2O2) on human renal tubular epithelial cell (HKC) and its effect on calcium oxalate (CaOxa) crystal crystallization time before and after the injury. Methods The injury degree of HKC by H2O2 was measured by detecting the cell survival rate and the concentration change of malonaldehyde (MDA). CaOxa crystallization was investigated by scanning electron microscopy (SEM). Results Control cells induced only a small amount of calcium oxalate dihydrate (COD) crystals, while the injured cells not only induced calcium oxalate monohydrate (COM) crystals, but also increased the number and aggregation of CaOxa crystals. After incubating with CaOxa supersaturated solution, the control group HKC cells could be injured as well. Conclusions H2O2 can cause oxidative damage on HKC. The injured HKC promotes the nucleation and aggregation of COM crystals. In the body environment, the long-term presence of crystals in urinary tract is a risk factor for stone formation.