Comparison of 3H-TdR and 125Ⅰ-UdR incorporation on the proliferation effect of lymphocytes
10.3760/cma.j.issn.0254-5098.2010.06.008
- VernacularTitle:3H-TdR与125Ⅰ-UdR掺入淋巴细胞增殖的比较
- Author:
Jianmei WAN
;
Youjiu ZHANG
;
Wo FAN
;
Ran ZHU
;
Ping NING
;
Fengmei CUI
- Publication Type:Journal Article
- Keywords:
Lymphocytes;
Lymphoma cells;
3H-TdR;
125Ⅰ-UdR;
Incorporating fraction
- From:
Chinese Journal of Radiological Medicine and Protection
2010;30(6):658-660
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the incorporation method of 3H-TdR and 125Ⅰ-UdR on determining the proliferation effect of lymphocytes. Methods The proliferation effects of lymphocyte and Daudi lymphoma cells were estimated by 3H-TdR and 125Ⅰ-UdR incorporation. Results The incorporating fraction of 3H-TdR and 125Ⅰ-UdR into lymphocyte was 20.95% ± 1.06% and 1.00% ±0.04%,respectively, and the incorporating fraction for the lymphoma cells was 29. 94% ± 4. 10% and 6. 02% ±0. 73% ,respectively. The incorporation fractions of 3H-TdR into lymphocyte and lymphoma cells were much higher than those of 125Ⅰ-UdR, but the incorporating fractions of 3H-TdR or 125Ⅰ-UdR into the lymphoma cells were much higher than those of lymphocytes. Conclusions For lymphocytes, 125Ⅰ-UdR cannot substitute 3H-TdR as a tracer agent. But for lymphoma cells, whether 125Ⅰ-UdR could be replace 3H-TdR or not needs further research.
