Effect of Dahuang Fuzi decoction on alveolaur epithelial barrier in rats with lung injury with severe acute pancreatitis
10.3760/cma.j.issn.1671-0282.2011.02.010
- VernacularTitle:大黄附子汤对重症急性胰腺炎肺损伤大鼠肺泡上皮屏障功能的影响
- Author:
Xiaoguang LU
;
Xin KANG
;
Libin ZHAN
;
Dianbo GONG
;
Li LIU
;
Zhiwei FAN
;
Lizhi BAI
;
Honggang PANG
;
Limin KANG
;
Chunyang JI
;
Xiaozhou WANG
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis;
Acute lung injury;
Alveolaur epithelial barrier;
Dahuang fuzi decoction;
Lung epithelial permeability;
Alveolar liquid clearance rate;
Total lung water content;
Extravascular lung water content
- From:
Chinese Journal of Emergency Medicine
2011;20(2):151-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effect of Dahuang Fuzi decoction on alveolaur epithelial barrier in rats with lung injury with severe acute pancreatitis. Method Ninty-six health SD rats were randomly divided into three groups: sham operation group, SAP-ALI group, Dahuang Fuzi decoction group, and then according to the time point of sacrifice after operation, each group was subdivided into 3,6,12,24 hour subsets ( each, n = 8). After the belly of a rat in the sham operation group was cut open, the pancreas was flipped several times,and then a stoma was made in the jejunum to form its fistula. In the SAP-ALl group,1 mL/kg sodium taurocholate was reversely injected into the pancreatobile duct to establish the model of SAP, and then the jejimum fistula was performed. The SAP-ALI model in Dahuang Fuzi decoction group was treated by injection of 10ml of Dahuang Fuzi decoctionon into the fistula respectively. Blood was collected from heart to detect serum amvlase and endotoxin (ET) levels before the rat being executed. The lung histopathologic changes, pulmonary injury scores and wet/dry weight(W/D) ratios were observed after the rats were executed. The alveolar liquid clearance rate(ALCR), total lung water content (TLW), extravascular lung water content(EVLW) and alveolar epithelial permeability (AEP) were examined in 3,6, 12,24 h after injury.Results There was continuous increase of AEP,TLW and EVLW,as well as progressive reduction of ALCR compared with sham operation group at 3,6,12,24 h after operation. Compared with SAP-ALI group, there was continuous decrease of AEP,TLW and EVLW, and elevated of ALCR at 3,6,12,24 h after operation.Conclusions Dahuang Fuzi decoction can significantly reduce alveolaur epithelial barrier and degree of lung tissue of SAP-ALI rats by inhibiting the elevation of LPS and inflammation reaction.
