Effects of the serum in shock rats and shenfu injection on the endothelial cell protein C receptor
10.3760/cma.j.issn.1671-0282.2011.02.014
- VernacularTitle:休克血清及参附对内皮细胞蛋白C受体的影响
- Author:
Yueyue HUANG
;
Jingye PAN
;
Guanyang LIN
;
Jinbo ZHANG
;
Xiaole ZHANG
;
Haifeng JIN
;
Zhiqiang LI
;
Jie CHEN
;
Jihong MA
- Publication Type:Journal Article
- Keywords:
hemorrhagic shock;
Shenfu injection;
endothelial cell protein C receptor;
Human umbilical vein endothelial cells;
rat;
shock;
therapy;
protein
- From:
Chinese Journal of Emergency Medicine
2011;20(2):168-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of the serum from rats with hemorrhagic shock and Shenfu injection, on the expression of endothelial cell protein C receptor (EPCR) in human umbilical vein endothelial cells (HUVECs) cultured with rat serum. Method The soluble endothelial protein C receptor (sEPCR) in supernatant, the expression of EPCR mRNA and protein level of EPCR in HUVECs were detected by using enzyme linked immunosorbent assay ( ELISA), reverse transcription polymerase chain reaction (RT-PCR), and western bloting (WB) in normal control group, hemorrhagic shock serum (3 h, 12 h, 24h, 72 h) group, and Shenfu-treated (3 h, 12 h, 24 h, 72 h) group, respectively. Results The mean levels of sEPCR and the expression of EPCR mRNA were significantly higher in hemorrhagic shock serum (12 h, 24 h) group, and Shenfu -treated(24 h)group than those in normal control group (all P ＜0.01 ),the mean levels of sEPCR and the expressions of EPCR mRNA were significantly higher in Shenfu-treated ( 12 h) group than those in normal control group ( all P ＜0. 05 ), while the levels of protein were lower in hemorrhagic shock serum ( 12 h, 24 h) group and in Shenfu-treated(24 h)group than those in normal control group ( both P ＜0.01 ), and the level of EPCR protein was lower in Shenfu-treated( 12 h) group than that in normal control group ( P ＜ 0. 05) . The mean levels of sEPCR and the expressions of EPCR mRNA were significantly lower in Shenfu-treated ( 12 h, 24 h) group than those in hemorrhagic shock serum ( 12 h,24 h) group (all P ＜0.05), while the levels of EPCR protein were higher in Shenfu-treated ( 12 h, 24 h)group than those in hemorrhagic shock serum ( 12 h, 24 h) group ( P ＜ 0.05 ). Conclusions These data suggest that Shenfu injectio could affect the expression of EPCR mRNA and the level of EPCR protein, thereby it might be effective in prevention of development of hemorrhagic shock.
