Neuronal damage and neurite change in cell model of intractable epilepsy
10.3760/cma.j.issn.1006-7876.2011.03.013
- VernacularTitle:难治性癫痫细胞模型中神经元损伤及神经突起的变化
- Author:
Yuan WU
;
Xiuying LIU
;
Xuefeng WANG
;
Yulan TANG
;
Lu YU
;
Jie SU
;
Yuejuan WU
;
Meigang MA
- Publication Type:Journal Article
- Keywords:
Epilepsy;
Neurons;
Neurites;
Models,neurological
- From:
Chinese Journal of Neurology
2011;44(3):196-199
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the cell model of intractable epilepsy and to observe its neuronal damage and morphologic change of neurites.Methods The model was established by exposing hippocampal neurons to Mg2+ -free media for 3 hours on days 10 of culture.Expression of lactic acid dehydrogenase (LDH) in supernatant was measured as an index of neuronal damage.The morphologic change of neurons and neurites was observed by optical microscope and scanning electron microscope (SEM).Results Compared to the control group, level of LDH (U/L) was significantly increased in the model group at different time points (3 hours: 4.26 ± 1.28, 6 hours: 6.56 ±2.34 and 24 hours: 16.67 ±3.57, P <0.05).With time prolonging, release of LDH in the model group was gradually increased (F = 39.316,P <0.05).Under optical microscope, neurons of model group migrated closely to each other and neurite connections appeared to be gradually "reticulated" after Mg2+ -free media treatment for 24 hours; and the "reticulated" neurites connections become more obvious after 72 hours.Under SEM, neuronal membrane was rough and had several small depressions, neurites were interlaced in cluster.Conclusions Neuronal damage and morphologic change of neurites are verified in the cell model of intractable epilepsy.
