Evaluation and applicatipn of media for isolation of haemophilus
- VernacularTitle:嗜血杆菌分离培养基的评价与应用
- Author:
Dongke CHEN
;
Yunjian HU
;
Xiuzhen ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Laboratory Medicine
2001;24(1):28-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To select suitable media for increasing isolation rate of haemophilus.Methods Haemophilus was inoculated on seven types of media.The average growth index(GI) of Haemophilus was calculated and Haemophilus was isolated from 325 specimens of the respiratory tract and its isolation rate was compared with that media of brchoc and brchoc-V agare.Results The GI of media with 2% fresh yeast infusion and 50% of meat infusion was higher than that of those with no yeast and meat infusion.The GI of Haemophilus on BRchoc was higher than that of the BSchoc(P<0.001).The isolation rate of Haemophilus from 325 respiratory tract specimens on BRchoc-V was 77.2%,which was significantly higher than that on the medium BRchoc (32.0%).Conclusions BRchoc-V is a better medium for isolation of Haemophilus.This medium is effective to improve the isolation rate of Haemophilus.
