Rapid detection of human adenoviruses in clinical samples by magnetic bead separation and PCR amplification
- VernacularTitle:磁分离法和聚合酶链反应技术在腺病毒临床诊断中的应用
- Author:
Hanzhong WANG
;
Ming WANG
;
Yi ZHOU
- Publication Type:Journal Article
- From:
Chinese Journal of Laboratory Medicine
2001;24(1):37-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid,effective,convenient method for detecting human adenoviruses and other viral pathogens using magnetic bead separation and PCR amplification.Methods A biotinylated oligonucleotide primers were hybridized to adenovirus DNA in stool samples.Setreptavidin coated magnetic beads were then added to isolate the DNA-oligonucleotide hybrid.The procedure allows for the recovery of viral DNA suitable for amplification by polymerase chain reaction.Results Ten samples collected from clinical stool were detected; six of them were positive.Results indicated that this nucleic acid separation technology is very effective in concentrating and purifying adenoviruses DNA while removing PCR inhibitors in stool samples.It also effectively increase the sensitivity of PCR amplification.Conclusion This technique can rapidly,reliably detect adenoviruses in clinical samples,and it can be used to detect other viral pathogens.
