Roles of subretinal fluid on expressio n of bcl-2 oncoprotein of cultured human retinal pigment epithelial cells
- VernacularTitle:视网膜下液对培养的人眼视网膜色素上皮细胞、成纤维细胞内Bcl-2蛋白表达的影响
- Author:
Miaoqin WU
;
Jing WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Ocular Fundus Diseases
2001;17(1):58-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of subretinalfluid (SRF) with different grades of proliferative vitreoretinopathy (PVR) onｂcl-2 oncoprotein expression in retinal pigment epithelium (RPE) cells and fib roblast (FB)．Methods Using immunohistologicalstain ingtechniｑue and Westernblotting method to detect the expression of ｂcl-2 protein in RPE cells and FB under the stimulation of SRF．Res u ltsThe expression levels of ｂcl-2 increased in both types oｆcells to a certain extent compared with those of the control group 4 hours afterthe cells subjected to SRF； 36 hours later, the expression levels of ｂcl-2 o f experimental groups decreased more quickly thanthose of the controlgroup， and the controlgroup showed relatively higher ｂcl-2 protein levels at the end of observation． Conclusions SRF may stimulate the e xpression of ｂcl-2 in RPE cells and FB under culture at early stage, but accel arate the declining of ｂcl-2 protein levels a certain time after subjected toSRF．
