Applying PFGE to the investigation of molecular epidemiology of Enterobacter cloacae in nocosomal infections
- VernacularTitle:脉冲场凝胶电泳应用于医院感染阴沟肠杆菌的分子流行病学
- Author:
Lei ZHANG
;
Ping LI
- Publication Type:Journal Article
- From:
Chinese Journal of Microbiology and Immunology
2001;21(2):171-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a fast typing method with good reproducibility and discriminatory power for epidemiology investigation of Enterobacter cloacae. Methods A method combining pulsed field gel electrophoresis (PFGE) with low-frequency-cleavage restriction endonucleases was established to analysis DNA of Enterobacter cloacae. Cells were imbedded in low melting point agarose. After purification of DNA in situ, it was digested with low-frequency-cleavage restriction endonucleases in situ. The intact DNA was electrophoresed in pulsed field. Relatedness of isolates was demonstrated by comparison of its ‘fingerprints’. Results 17 clones were found among 28 Enterobacter cloacae isolated from nocosomal infections. Outbreak was not registered during the period of six-months (from Jan. 1999 to Jun. 1999). But two small clone transmission happened during this period. They were clone A and clone B, five strains were implicated respectively. Conclusion PFGE is a useful technique with good discrimination and reproducibility for investigating the epidemiology of Enterobacter cloacae in microbiology laboratory.
