ifferentiation inducing effect of Bifidobacterial surface molecules on human colonic cancer cells
- VernacularTitle:双歧杆菌表面分子诱导大肠癌细胞分化的研究
- Author:
Hong JIANG
;
Hong HU
- Publication Type:Journal Article
- From:
Chinese Journal of Microbiology and Immunology
2001;21(2):186-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the direct antitumor effect of several surface molecules of Bifidobacterium bifidum 1101 such as whole peptidoglycan (WPG), lipoteichoic acid (LTA), and polysaccharides (PS) on human colonic adenocarcinoma cell line LoVo. Methods MTT assay and flow cytometry (FCM) were used to evaluate the effect of Bifidobacterial surface molecules on the growth of LoVo cells. Morphological study, fluorescent polarimetry and differentiation-specific enzyme analysis were used to determine whether LTA treatment could induce the differentiation of the cells. Results LTA but not WPG or PS significantly inhibited the proliferation of LoVo cells in a dose and time dependent manner. Cell cycle analysis by FCM revealed that LTA induced changes in cycle distribution resulting in G0/G1 phase commitment. Morphological alterations observed by light and electron microscopy indicated maturity of cytoplasm and nucleus following treatment by 50μg/ml of LTA. Membrane fluidity was also altered, and intestinal alkaline phosphatase specific for the differentiation of LoVo cells roused dramatically during culture in LTA. It was further found that LTA elevated ［Ca2+］i which resulted from Ca2+ influx instead of the release of stored calcium. Conclusion These observations indicated that LTA was able to inhibit the growth of LoVo cells and induce the differentiation of the cells. Calcium influx may play an important role in this process.
