Attachment of Streptococcus pneumoniae to A549 cells induces tyrosine phosphorylation of host cell proteins
- VernacularTitle:肺炎链球菌粘附肺Ⅱ型上皮细胞触发宿主细胞酪氨酸蛋白磷酸化
- Author:
Yingjun YAN
;
Yibing YIN
;
Gefei KANG
- Publication Type:Journal Article
- From:
Chinese Journal of Microbiology and Immunology
2001;21(2):196-199
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potentiality of adhesion of S. pn to type Ⅱ pneumocyte (A549) in inducing tyrosine phosphorylation of A549 cell proteins and the function subcomponents involved in. Methods Labelled S. pn with FITC for the observation of kinetic characters of adhesion in vitro; The tyrosine phosphorylation of A549 cell proteins induced by S. pn was examined by immunohistochemistry and ELISA. Results The adhesion of S. pn to A549 cells was in a time-and dose-dependent fashion suggesting the specific interaction; Tyrosine phosphorylation induced by adhesion was dose-and time-dependent and was mediated by the surface proteins of S. pn. Conclusion S. pn adheres to A549 cells and triggers the signal cascade in which the surface proteins of S. pn play a vital role.
