Effects of dexamethasone on VEGF-C and VEGFR-3 in primary culture in vitro of laryngeal squamous carcinoma tissue
- VernacularTitle:地塞米松对喉鳞状细胞癌血管内皮生长因子-C及血管内皮生长因子受体-3表达的影响
- Author:
Jinyuan WANG
;
Binquan WANG
;
Haili ZHANG
- Publication Type:Journal Article
- Keywords:
Laryngeal neoplasms;
Dexamethasone;
Cell culture techniques;
Vascular endothelial growth factor-C;
Vascular endothelial growth factor receptor-3
- From:
Cancer Research and Clinic
2008;20(3):168-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of VEGF-C,VEGFR-3 gene in primary culture in vitro of laryngeal squamous carcinoma tissue by dexamethasone (DEX). Methods Applying the technique of Drimary tissue cuiture in vitro,twelve specimens,which were identified pathologically as laryngeal squamous cell carcinoma,were cultured.After succeeded culture of twelve specimens,the cells were divided in to two groups.One continued to cuhum,another gave 10-7 mol/L of DEX.VEGF-C and VEGFR-3 gene express in 12 cases of cell were examined by reverse transcription polymerase chain reactions(RT-PCR).Results The frequency of VEGF-C(83%) and VEGFR-3(58%) in primary culture in wtro of laryngeal squamous carcinoma tissue were higher than that of the primary culture in vitro of laryngeal squamous carcinoma tissue by dexamethasone (DEX) (25%,8%P＜0.05).Conclusion The expressions of VEGF-C and VEGFR-3 in Drimary culture in vitro of laryngeal squamous carcinoma tissue by DEX are decreased.It may provide guide to apply DEX in clinic laryngeal squamous carcinoma chemotherapy and laryngeal squamous carcinoma laryngeal obstruction.
