The adhesion of Neisseria gonorrhoeae to COS-1 cells mediated by human CEACAM1
- VernacularTitle:hCEACAM1介导淋病奈瑟菌对COS-1细胞的黏附
- Author:
Guocai LI
;
Jinsong WANG
;
Litian ZHU
;
Hongmei JIAO
;
Mingchun JI
- Publication Type:Journal Article
- Keywords:
Neisseria gonorrhoeae;
hCEACAM1;
Gene transfection;
COS-1 cells;
Adhesion
- From:
Chinese Journal of Microbiology and Immunology
2008;28(2):166-169
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of human carcinoembryonic antigen-related cellular adhesion molecule 1 (hCEACAM1)in mediating the specific adhesion of N. gonorrhoeae to its human host cells.Methods A recombinant eukaryotic expression vector pCDPGICEA1 was constructed by putting hCEACAM1 cDNA behind both hCD46 promoter and rabbit β-globulin intron 2,and with which,the COS-1 cells were transfected. Following G418 selection, the COS-1 cells expressing hCEACAM1 were sorted out with flow cytometry. The adhesion of N. gonorrhoeae to the gene transfected COS-1 cells was analyzed with bacterial binding assay. Results hCEACAM1 cDNA could be expressed effectively under the direction of hCD46 promoter and rabbit β-globulin intron 2,and N. gonorrhoeae could adhere to COS-1 cells expressing hCEACAM1. Conclusion hCEACAM1 can mediate the adhesion of N. gonorrhoeae to animal originated COS-1 cells. thus its transgenic mice may be used as a novel animal model for studying N. gonorrhoeae infection.
