The investigation of the mechanism enhancing killing activities of tumor cell-specific cytotoxic T lymphocyte by IL-4 gene modification
- VernacularTitle:人IL-4基因修饰增强瘤细胞特异性CTL杀伤活性机制初探
- Author:
Chengrong LI
;
Jingrong FU
- Publication Type:Journal Article
- From:
Chinese Journal of Microbiology and Immunology
2001;21(3):292-295
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of enhancing killing activities of tumor cell-specific cytotoxic T lymphocyte (CTL) by IL-4 gene modification. Methods IL-4 gene was introduced into human hepatocellular carcinoma cell HepG2 cells using retroviral vector pL-IL-4-SN. Wild HepG2 and IL-4 gene-modified tumor cells were used to induce CTL responses, and cellular surface molecules were evaluated using flow cytometry. Results The killing activities of tumor cell-specific CTL in IL-4 gene-modified groups were increased from (11.2±2.7)% to (72.5±12)%, which was about seven fold higher than CTL killing activities induced by HepG2 cells (P<0.01). High levels of expression of MHC class Ⅱ antigens as well as the co-stimulatory molecules B7-1 and ICAM-1 were found in IL-4 gene-transferred tumor cells. The expression of MHC class Ⅰwas not affected by IL-4 gene modification. The expression of cell surface molecules induced by IL-4 gene-modified tumor vaccines were completely abrogated through using anti-IL-4 McAb. A significant increase of IL-2 secretions was found during the induction of CTL responses when IL-4 gene-modified tumor cells were used as stimulatory cells. IL-2 secretion and CTL responses were inhibited by anti-IL-4 or anti-surface molecule McAb. Conclusion IL-4 gene modification enhanced tumor cell-specific CTL killing activities through induction of the expression of cellular surface molecules and facilitation of IL-2 secretion.
