Amino acid mutatios of gp41 2F5 and 4E10 neutralizing epitopes of 92 HIV-1 infected individuals and AIDS patients in China
- VernacularTitle:中国92例HIV/AIDS患者HIV-1 gp41 2F5和4E10中和抗体表位变异研究
- Author:
Xiaoli ZHANG
;
Xiaoxu HAN
;
Di DAI
;
Xihua DONG
;
Mingjia BAO
;
Bin ZHAO
;
Yongjun JIANG
;
Yanan WANG
;
Zining ZHANG
;
Liping ZHOU
;
Hong SHANG
- Publication Type:Journal Article
- Keywords:
HIV-1;
gp41;
Neutralizing epitope;
2F5;
4E10
- From:
Chinese Journal of Microbiology and Immunology
2008;28(3):258-263
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the amino acid mutations in neutralizing antibody 2F5 and 4E10 conserved epitopes ELDKWA and NWFDIT of HIV-1 membrane proximal external region(MPER)in 92 HIV-infected individuals and AIDS patients in China,and to provide a basis for the neutralizing antobodies immunotherapy and a design of vaccines. Methods Nest-PCR methods were used to amplity genes of the HIV-1 env gp41 region.The amplified fragments were sequenced by double-deoxygen terminal method and translated into amino acids for analysis.The mutations of 2F5 and 4E10 neutralizing epitopes were identified by comparison with the epitopes reference data in HIV-1 Sequence Database.Results There were mutations on both 2F5 and 4E10 neutralizing epitopes.2F5 conserved neutralizing epitopes major mutations tocused on E662A(14.1%),K665S(17.4%),A667K(16.3%),and 4E10 conserved neutralizing epltopes major mutations included N671S(13.0%),D674S(3.3%),T676S(16.3%).The mutation rates of 2F5 and 4E10 epitopes were significanfly different between CRF_B'C-clade and B'-clade(P<0.05).The mutata rates of CRF_B'C-clade were higher than that of CRFOI_AE-clade in 2F5 epitopes(P<0.05).The mutation rates of B'-clade in 4E10 eiptopes showed significant difference in slow progressors,HIV-infected individuals and AIDS patients,respectively(P<0.05).Conclusion The HIV-1 patients in China are demonstrated diversified mutations in 2F5 and 4E10 neutralizing epitopes.The mutation degrees of amlno acids in conserved neutralizing epitopes are different in different subtypes.There may be a correlation between neutralizing epitopes mutations of 4E10 with disease progression.
