Generation of cold-adapted, attenuated reassortant human influenza A virus with eight-plasmid system
- VernacularTitle:用8质粒病毒拯救系统产生冷适应减毒的重组A型人流感病毒的研究
- Author:
Penghui YANG
;
Xinfu SHI
;
Yan YAN
;
Deyan LUO
;
Yu ZHANG
;
Li XING
;
Jinxue LONG
;
Xiufan LIU
;
Xiliang WANG
- Publication Type:Journal Article
- Keywords:
Influenza virus;
Reverse genetics;
Eight-plasmid system;
Reassorted influenza virus
- From:
Chinese Journal of Microbiology and Immunology
2008;28(2):139-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a technical platform of reverse genetics based on the 8 plasmid.virus rescue system of cold-adapted influenza virus strain. Methods The cold-adapted, temperature sensitive, live attenuated influenza virus strain A/AnnArbor/6/60(H2N2)was chosen as the master donor virus(MDV)for rescue research,and its six internal gene fragments PB2,PB1,PA,NP,M and NS were artificially synthesized. Meanwhile, five amino acid mutations have been introduced as tags. Six fragments were ligated with modified pAD3000 for the construction of rescue plasmid. Six transcription/expression plasmids(pMDV-A-PB2,pMDV-A-PB1,pMDV-A-PA,pMDV-A-NP,pMDV-A-M,and pMDV-A-NS)were obtained, and their sequences were accurate. Results The reassorted virus named as rMDV-A contains HA and NA gene segments derived from PR8 strain along with six gene segments,PB2,PB1,PA,NP,M and NS,from MDV. The COS-1 cells were co-transfected with eight recombinant plasmids. The results showed that a cold-adapted, attenuated reassortant influenza A virus with hemagglutination activity was rescued successfullv bv"6+2" combination of MDV and PR8, and the allanotoic fluid of the injected eggs gave a posigenes of A/AA/6/60 used as backbone has provided experimental materials for further research on the gene function and novel vaccine candidate of cold-adapted, attenuated human influenza virus.
