Rapid detection of chromosomal aneuploidies by multiplex ligation-dependent probe amplification
- VernacularTitle:多重探针连接依赖式扩增快速检测染色体非整倍体异常
- Author:
Xinping FAN
;
Lirong WANG
;
Bai XIAO
;
Jingzhong LIU
;
Shuying GAO
;
Lin ZHANG
;
Ying ZHANG
;
Ying GU
- Publication Type:Journal Article
- Keywords:
Aneuploidy;
Down syndrome;
Prenatal diagnosis;
Oligonucleotide probes;
Polymerase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2008;31(1):77-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test whether multiplex ligation-dependent probe amplification(MLPA)could be used for the prenatal detection of the most common aneuploidies of chromosomes 13,18,21,X,and Y.Methods 34 cases including 22 blood samples(12 with trisomy 21,1 with monosomy X,one male witll extra Y and 8 healthy persons),4 cord blood samples with Down syndrome and 8 amniotic fluid samples ( 1 with trisomy 21 and 7 normal fetuses)were recruited into this study.All samples were confirmed by karvotype analysis. DNA was extracted from blood and amniotic lysate was incubated with proteinase K.MLPA was used to determine the relative copy numbers.Results The resuhs were available within 48 h and were concordant with karyotype analysis in all but one case of amniotic fluid that was suggested to be triploid sample 69,XXY by MLPA or contaminated by maternal blood.This sample actually was found containing a number of red blood cells after centfifugation in test. In total,the concordance rate with clinical characteristics was 97.1%.The Ratio values of 13,18,21,X in normal samples were approaching 1.0 except chromosome Y having slightly higher variation in relative copy number.The difference of ratio means between the normal and trisomy 21 samples was statistically significant by one-way ANOVA(F=298.906.P=0.000).Conclusion Computer assisted MLPA with high sensitivity is a rapid,simple,automatic and reliable method for detection of common chromosomal aneuploidies.
