Impact of calcium dobesilate on the expression of CD34 and von Willebrand factor in renal tissue of chronic aristolochic acid nephropathy rats
- VernacularTitle:羟苯磺酸钙对慢性马兜铃酸肾病大鼠肾组织CD34和血管性血友病因子表达的影响
- Author:
Wei GUO
;
Jiangmin FENG
;
Wei LI
;
Jie ZOU
;
Li SUN
;
Lining WANG
- Publication Type:Journal Article
- Keywords:
Calcium dobesilate;
Aristolochia acid nephrapathy;
Microvessel
- From:
Chinese Journal of Nephrology
2008;24(3):185-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of calcium dobesilate on the expression of CD34 and VOH Willebrand factor(vWF)in peritubular capillary (PTC)in renal tissues of rats with chronic arisolochic acid nephropathy(CAAN)and to explore the probable mechanism concerned.Methotis Sixteen Wistar rats were infused with Caulis aristolochia manshuriensis decoction for 12weeks,then randomly divided into 2 groups.NX group rats(n=8)were infused with distilled water and treatment group(n=8)with calcium dobesilate at the following 4 weeks solely.Control group rats(n=8)were infused with distilled water for the 16 weeks.At week 16,all the rats were sacrificed.Specimens of blood and urine were collected to detect the blood urea nitrogen (BUN),serum creatinie(Scr)and urine protein.HE and Masson staining was used to observe the pathology of the kidney.Immunohistochemistry was used to detect the expression of CD34 and vWF.Results Urinary protein,Scr and BUN in calcium dobesilate treatment group were much lower than those in NX group (P＜0.05).The A value of CD34+ increased significantly in calcium dobesilate treatment group[(16.72±4.17)×103]compared with NX group[(3.19±1.40)×103]at week 16(P＜0.01).The A value of vWF+decreased in calcium dobesilate treatment group[(10.16±1.68)X103]compared with NX group[(18.66±4.65)x103]at week 16(P＜0.01). Conclusion Calcium dobesilate can increase the expression of CD34 and the density of peritubular capillary(PTC)in renal tissues of CAAN rats,and reduce the expression of vWF and the formation of microthrombosis.
