Determination of chlorogenic acid in xiaoaiping injection in human plasma by reversed-phase high performance liquid chromatography
- VernacularTitle:反相高效液相色谱法测定人血浆中消癌平注射液的绿原酸浓度
- Author:
Keli YAN
;
Xiuqing ZHU
;
Li ZHAO
;
Yu BAI
- Publication Type:Journal Article
- Keywords:
Plasma concentration;
Chlorogenic acid;
Chromatography,high pressure liquid;
Xiaoaiping injection
- From:
Cancer Research and Clinic
2008;20(3):158-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC method for the determination of chlorogenic acid in xiaoaiping injection in human plasma.Methods The analytical column was packed with Hypersil ODS C18 (250 mm×4.6 nm,5 μm).The mobile phase was acetonitrile-0.4% phosphoric acid solution(13:87).The flow rate was 1 ml/min,and detection wave length was set at 327 nm.Results The linear ranges were 2.5～60.0 mg/L chlorogenic acid in human plasma,with the correlation coefficients of 0.9996.The Low limit of detection was 0.25 μg/ml.The method and extraction recovery were 100.1%～102.6%and 78.6%～80.3%.The relative standard deviation value for the within-day and between-day precision were less than 3.9%.Conclusion The method is simple,sensitive for the determination of chlorogenic acid in xiaoaiping injection in human plasma.
