The study about apoptosis of STI571 on NB4 in vitro
- VernacularTitle:伊马替尼诱导早幼粒细胞白血病细胞株NB4凋亡的实验研究
- Author:
Zhijun ZHANG
;
Xiujuan ZHAO
;
Liangming MA
;
Zhenhua QIAO
- Publication Type:Journal Article
- Keywords:
STI571;
Apoptosis;
NB4
- From:
Cancer Research and Clinic
2007;19(z1):8-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study was designed to explore the influence about apoptosis of STI571 on NB4.Methods After NB4 cells were treated with STI571 in different concentration(0.5,1.0,5.0umol/L)at indicated time(24h,48h,72h)(1)morphological observation:To determine cell morphology by light microscope.(2)MTT assay:Inhibition of proliferation was measured with a colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny tetrazolium bromide(MYa3 assay.(3)Flow cytometric analysis:The expression of survivin and smac were measured on NB4 by FCM.Results Morphological observation showed characteristic apoptosis changes.MTT assay showed that STI at (0.5,1.0,5.0 μmol/L) range for 24 h,48 h and 72 h can markedly inhibit the proliferation of NB4 cells in a dose-dependent manner as well as a time-dependent manner(P＜0.05).FCM showed the expression of survivin decrease and smac increase.Conclusion STI571 In Vitro inhibits proliferation of NB4.
