Dual effect of Molt-4 cell lines onset with Roscovitine
- VernacularTitle:Roscovitine对Molt-4细胞株作用的双重效应
- Author:
Haocheng LONG
;
Xia GAO
;
Zhijian PAN
- Publication Type:Journal Article
- Keywords:
Roscovitine;
Apoptosis;
G2/M arrest;
Molt-4 cells;
FACS analysis
- From:
Cancer Research and Clinic
2007;19(z1):1-3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Mlot-4 cells onset with Roscovitine (ROSC)as some Cyclin-dependent kinases(CDK),inhibitor.Methods The logarithmic growth phase Molt-4 cells treated with ROSC at a final concentration ranging between 1～20 μmol/L and harvested in different time point,DNA assay of single cells by flow cytometry was used to detect the effect of cell cycle arrest and Annexin-V/FITC assay was used to detect the effect of cell apoptosis. Results It showed that ROSC exerted strong inhibitory effect on proliferation and cell cycle progression of Molt-4 Accumulation of G2/M arrested cells starting 6 h after onset of 10 μmol/L and 20 μmol/L ROSC;at the same time,the cell apoptosis of Molt-4 would be detected,According with the time and concentration changing,the cell apoptosis rate would rise.Conclusion It is concluded that Roscovitine(ROSC)as some Cyclin-dependent kinases(CDK),inhibitor,It has dual effects to Molt-4 cells,not only the effect of cell cycle arrest but also the effect of cell apoptosis.
