The express of angiotensin Ⅱ in lungs during bilateral femoral arteriovenous shunt for extracorporeal membrane oxygenation
- VernacularTitle:Ang Ⅱ在pECMO转流早期组织中的表达
- Author:
Peng XIA
;
Xigang GENG
;
Yang YAN
- Publication Type:Journal Article
- Keywords:
Extracorporeal membrane oxygenation;
Angiotensin Ⅱ;
Immunohistochemistry;
Respiratory insufficiency
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2008;24(2):119-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the change of angiotensin Ⅱ in the applications of pumpless ECMO, and its effect on prognosis of the acute respiratory failure. Methods The study was performed in ten dogs [ weight 18 - 35 kg, mean weight ( 23.4 ± 4.7 ) kg].A respiratory failure animal model was established end then was treated by bilateral femoral artery-venous ECMO. Collection right atriurn blood end constitution of lung at different time (before ECMO, during ECMO 1h, 2h, 3h, 4h). Angiotensin Ⅱ content in blood and lung homogenate was detected by radio-immunity mothod. Angiotensin Ⅱ expression loci in lung were detected by immunohistochemistry mothod. Results Angiotensin Ⅱ content in plasma was decreased at completion of the model, it was back up at 1 hour and reached the peak at 3 hours, then it slowly declined. Angiotensin Ⅱ content in lung homogenate increased at the beginning, peaked by 2 hours, end then it decreased. Loci angiotensin Ⅱ in lung by immunohistochemistry were expressed in most of epithelial cells cytoplast of bronchiole dissepimont, smooth muscle cell cytoplast of small blood vessel around it end a few macrophage cytoplasts during the model time..Conclusion It is valuable to measure engiotensin Ⅱ in blood through the bilateral femoral artery-venous ECMO, because it can reflect angiotensin Ⅱ in lung and the resume of lung in certain degree.
