Photoprotection by the selective COX-2 inhibitor NS398 against UV-mediated damage in keratinocytes
- VernacularTitle:环氧化酶-2选择性抑制剂NS398对紫外线照射损伤人角质形成细胞的保护作用
- Author:
Fang YAN
;
Hong LIANG
;
Tiechi LEI
- Publication Type:Journal Article
- Keywords:
Cyelooxygenase-2;
Ultraviolet;
HaCaT cell;
NS398;
Reactive oxygen species
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2008;14(2):126-128
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate photo-protective effect of NS398 on above cells damaged from UV irradiation.Methods HaCaT cell and HaCaT keratinocytes were incubated in the culture medium supplemented with different concentration of NS398 for 2h before diffierent dosages of UV irradiation.MTT assay was used to detect cell proliferation and cellular activity:Fluorospectrophotometer assay was used to measure the change of ROS level in the cells which were UV-mediated.Resuits After irradiation with UV,proliferation and cellular activity of HaCaT cells were decreased(P＜0.05);NS398 reduced the generation of ROS in cells induced by UV irradiation apparently and the cell apoptotic rate was dependent on the concentration of NS398. Conclusion NS398 might play an important role in the treatment of UV irradiation injury by decreasing the generation 0f ROS in cells after UV irradiation and increasing the survival ability of HaCaT cells.
