Disfunction of the dendritic cells from the aquaporin 5 knockout mice
- VernacularTitle:水通道蛋白5基因对小鼠树突状细胞功能影响的研究
- Author:
Guifang WANG
;
Gusheng TANG
;
Chunling DONG
;
Qian SHEN
;
Chunxue BAI
- Publication Type:Journal Article
- Keywords:
Aquaporin 5;
Dendritic cell;
Gene knockout;
Co-stimulating molecular
- From:
Chinese Journal of Microbiology and Immunology
2008;28(4):299-302
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of aquaporin 5(AQP5)gene to the differentiation and development of bone marrow-deriued dendritic cells(BMDC).Methotis The DCs obtained from the bone marrow of the AQP5-/- and the wild type(WT)mice were cultured and induced to maturation by LPS.The phenotype of the two types DCs were assayed by FACS,and the phagocytosis ability Was assayed by co-incubating with FITC-conjucted ovalbumin.Results Contrast to the DCs from the WT mice,the DCs from the A9P5-/-mice showed reducing positive rate of expression of the co-stimulating molecular such as CD40,CD80.CD86.And the phagoeytosis ability of DCs from the AOP5-/-mice was lower than that of the WT mice.The relationship of the phagncytosis rate to the time from the AOP5-/- mice was different from that of the WT mice.The stimulating ability of the AOP5-/-DCs was lower than that of the WT mice.Conclusion Water transmembrane moving mediated by AQP5 iS very important to the normal function of DCs.The certain mechanism of the signal moleeular is to be determined. Aquaporin 5;Dendritic cell;Gene knockout;Co-stimulating molecular
