Screening of antigenic epitope of neutrophil-activating protein of Helicobacter pylori by phage display peptide Hbrary
- VernacularTitle:应用噬菌体随机肽库技术筛选幽门螺杆菌中性粒细胞激活蛋白的抗原表位
- Author:
Jun LUO
;
Yan LI
;
Jianping LAI
;
Min LONG
;
Wenbing ZHANG
;
Beiguo LONG
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Neutrophil-activating protein;
Epitope;
Phage peptide library
- From:
Chinese Journal of Microbiology and Immunology
2008;28(4):357-362
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify epitope of neutrophil-activating protein(NAP) of Helicobacter pylori(Hp).Methods Using the mouse monoclonal antibodies against NAP as selective molecular and immunoscrcening phage-display random 7-peptides library.The positive clones were sequenced and analyzed.Phage clones were chosen to immunize mice and to evaluate the potential of phagotopes as effective vaccines.Results One mimotope(FAHLATQ)showed a good match with the NAP at 140-143 AA(AHLA)and the serum of mice induced by the phage clone clearly recognized NAP.Conclusion This study suggests thatthe antigenic epitope could be mapped through screening the phage-display peptide library with monoclonalantibody and a mimotopo of NAP providing an ahernative approach for the diagnosis and development of avaccine for Hp.
