The inhibitory effect of pigment epithelium-derived factor on migration of gliomas
- VernacularTitle:色素上皮细胞衍生因子与胶质瘤侵袭的相关性
- Author:
Tao ZHANG
;
Ming GUAN
;
Yuan Lü
- Publication Type:Journal Article
- Keywords:
Glioma;
Neoplasm invasiveness;
Eye proteins;
Nerve growith factor;
serpin
- From:
Chinese Journal of Laboratory Medicine
2008;31(2):200-203
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the impact of pigment epithelium-derived factor(PEDF) on the migration of the glioma cells.Methods PEDF(100 ng/ml)was added to U87 cells(U87PFDF),and VEGF of 0.25μg/ml was added to U87PEDF+VEGF while U87 cells as control(U87con).The cell migratory assav was used tbr evaluating its inhibitory migration rate of PEDF.Real time RT-PCR was used for evaluating the expression of Laminin-8 gene.Results The number of migratory cells was higher than those with added PEDF,and the inhibitory rate of migratory was 38% even in the presence of VEGF.Real time RT-PCR revealed that the mRNA expression levels of α4,β1,γl were(1.043±0.090),(0.823±0.012).(0.762±0.05) copy/μl,which were higher than those treated by PEDF(0.633±0.004),(0.442±0.005).(0.424±0.002)copy/μl,respectively (P＜0.05).Conclusion PEDF could decrease the migmtory capacity of the glioma cells even in the presence of VEGF because of the regulation of Laminin-8 in part.
