Comparative study of the adjuvanticity of type-A,B and C CpG-ODN in mice
- VernacularTitle:三种类型CpG-ODN作为蛋白亚单位疫苗佐剂的比较研究
- Author:
Zhongyang ZHANG
;
Xiaoxiao WANG
;
Chunsheng HAO
;
Xiaoxia ZHENG
;
Lingnan BU
;
Honglin XU
- Publication Type:Journal Article
- Keywords:
CpG-ODN;
Hepatitis B surface antigen;
Adjuvant
- From:
Chinese Journal of Microbiology and Immunology
2008;28(4):368-372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the adjuvanticity of type-A,B and C CpG-ODN in mice.Methods Three types of CpG-ODN were identified through in vitro stimulation of murine splenocytes with various CpG-ODN.BALB/c mice were immunized with HBsAg,together with different types of CpG-ODN,and antigen-specific IgG.IgG1 and IgG2a titers were measured 4 weeks later by indirect ELISA.Resuits Compared with the control group.all 3 types of CpG-ODN enhanced humoral immune response to HBsAg.However,type-B and C CpG-ODN induced much higher levels of antigen-specific IgG and IgG2a than type A CpG-ODN.Type-C CpG-ODN induced a similar TH 1-biased immune response as type-B CpG-ODN,revealed by decreased IsG1 to IgG2a ratio.In contrast,although type-A CpG-ODN increased IgG titers,it did not switch the balance between TH1 and TH2 immune responses.Conclusion All 3 types of CpG-ODN can enhance the humoral immune response to vaccines,but their aaiuvanticity could be mediated through different mechanisms.
