Impact of parathyroid hormone on transition and connective tissue growth factor expression of human renal tubular epithelial cells
- VernacularTitle:甲状旁腺激素对人近曲小管上皮细胞转分化及分泌结缔组织生长因子的影响
- Author:
Yunshan GUO
;
Weijie YUAN
;
Hanyang YE
;
Peng FU
;
Xiaobin MEI
;
Xiaoli ZHAN
;
Ling LIU
- Publication Type:Journal Article
- Keywords:
Parathyroid hormone;
Kidney tubules;
Epithelial cells;
Fibrosis;
Connective tissue growth factor
- From:
Chinese Journal of Nephrology
2008;24(5):332-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of parathyroid hormone (PTH) on the transition and connective tissue growth factor (CTGF) expression of human renal proximal tubular epithelial cell line HK-2 . Methods The expression of CTGF mRNA and protein of HK-2 cells were measured by real time RT-PCR and Western blot respectively . The effect of PTH on the phenotypic transformation of HK-2 cells was examined by light microscopy . The expression of α-smooth muscle actin (α-SMA) in HK-2 cells was detected by immunofluorescence . Results Basal level of CTGF mRNA and the protein expression were detected in HK-2 ceils . PTH upregulated the expression of CTGF mRNA and protein with the maximal response at the concentration of 10-10 mol/L and the best stimulating time was at 72 h . After exposure to PTH (10-10tool/L) for 12 hours, the highest level of luciferase activity was 1 .96 fold as compared to control (1 .888±0 .078 vs 0 .989±0 .030, P＜0 .01 ) . Untreated cells showed negligible expression of ±-SMA,whereas ±-SMA expression was significantly increased in cells treated with PTH . Conclusion PTH up-regulates CTGF expression and induces transition of HK-2 cells .
