Effect of fosinopril on the expression of NADPH oxidase p22phox mRNA in the kidneys of diabetic rats
- VernacularTitle:福辛普利对糖尿病大鼠肾脏NADPH氧化酶p22phox亚基mRNA表达的影响
- Author:
Yanqiang PENG
;
Yulian JI
;
Zongpei JIANG
;
Xueqing YU
;
Haiyan ZHANG
- Publication Type:Journal Article
- Keywords:
Angiotensin-converting enzyme inhibitor;
NADPH oxidase;
Oxidativestress;
Diabetes mellitus;
Extracellular matrix
- From:
Chinese Journal of Nephrology
2008;24(5):343-348
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of fosinopril on the expression of NADPH oxidase subunit p22phox mRNA and the extracellular matrix (ECM) accumulation in the kidneys of rats with diabetes mellitus . Methods Diabetic rats induced by streptozotocin were randomly divided into control group(DM group) and fosinopril group (fosinopril 10 mg'kg-1·d-1) (DM+Fosin group) and treated for 12 weeks . Expression of p22phox mRNA of NADPH oxidase in kidneys was measured by RT-PCR . The expression of fibroneetin was studied by immunohistochemistry and matrix metalloproteinases 9 activity was detected by Zymography . Meanwhile, the kidney hypertrophy index, serum creatinine level and 24-hour urinary protein excretion were evaluated . Results The expression level of p22phox mRNA in the kidneys of DM+Fosin group rats was decreased by 45% than that of DM group at week 4 (P＜0 .05) . At week 8 fosinopril significantly decreased the expression of glomerular and tubulointerstitial fibronectin by 52,5% and 42 .9% respectively (P＜0 .05), while increased MMP-9 activity by 29 .6% (P＜0 .05) compared with DM group . Fosinopril significantly decreased 24-hour urinary protein excretion of diabetic rats from week 8 . Serum creatinine level, 24-hour urinary protein excretion and kidney hypertrophy index were significantly decreased by 35 .9%, 50 .2% and 17 .2% in rats of DM+Fosin group than those of DM group at week 12 (P＜0 .05) . Fosinopril did not affect blood sugar significantly . Conclusion Fosinopril has beneficial effect on diabetic nephropathy partly through inhibiting the expression of NADPH oxidase p22 phox mRNA .
