Determination of serum total glycerol by isotope dilution liquid chromatography tandem mass spectrometry
- VernacularTitle:同位素稀释液相色谱串联质谱法测定血清总甘油
- Author:
Weiyan ZHOU
;
Lei LIU
;
Chuanbao ZHANG
;
Chunhua SUN
;
Xin HU
;
Hongxia LI
;
Jun DONG
;
Shu WANG
;
Ziyu SHEN
;
Wenxiang CHEN
- Publication Type:Journal Article
- Keywords:
Glycerol;
Triglycerides;
Chromatography,liquid;
Spectrum analysis,mass;
Isotope labeling;
Carbon isotopes
- From:
Chinese Journal of Laboratory Medicine
2008;31(3):254-257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for measuring serum total glycerol by isotope dilution (ID)liquid chromatography tandem mass spectrometry(LC/MS/MS).Methods Serum samples were mixed with [13C3]-glycerol(internal standard)and treated with alcoholic potassium hydroxide to hydrolyze serum glycerides to glycerol.The natural glycerol and the internal standard were benzoylated with the Schotten-Baumann reaction and the benzoates were analyzed by LC/MS/MS.Results The correlation coefficients between the peak area ratios and glycerol concentrations were 0.999 9 and higher.The withinrun coefficients of variation(CV)for serum total glycerol analysis averaged 0.94%(ranged 0.21%～2.62%)and the total CV 1.15%(0.62%～2.00%).Results on certified reference materials(SRM 909b Level Ⅰ and LevelⅡ;GBW 09146 and GBW 09147)showed an averaged bias of 0.20%(-0.20%～1.06%).Conclusions An ID-LC/MS/MS method for serum total glycerol has been developed.The method iS highly specific and precise and may be used as a candidate reference method for serum total glycerol measurements.
