Preparation of serum total cholesterol,total glycerol,free glycerol and triglycerides reference materials
- VernacularTitle:血清总胆固醇、总甘油、游离甘油和甘油三酯标准物质的研制
- Author:
Jun DONG
;
Hongxia LI
;
Yong MAN
;
Hanbang GUO
;
Wenxiang CHEN
;
Shu WANG
;
Bei XU
;
Ruifeng XU
;
Dazhou CHEN
;
Baosheng CHEN
- Publication Type:Journal Article
- Keywords:
Cholesterol;
Glycerol;
Triglycerides;
Reference values;
Blood chemical analysis
- From:
Chinese Journal of Laboratory Medicine
2008;31(3):276-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare serum cholesterol and triglycerides reference materials.Methods Blood units were collected from healthy donors and the sera separated and screened for cholesterol and triglycerides levels.Four serum pools were prepared by pooling sera grouped according to cholesterol and triglyceride levels.The materials were tested for homogeneity and stability and their values for total cholesterol,total glycerol and free glycerol and triglycerides were assigned by HPLC methods.Results The materials were tested to be homogeneous and stable for at least 4 years at-20℃.The certified values (reference value±expanded uncertainty)of the 4 materials for total cholesterol were(5.110±0.065) mmol/L,(4.761±0.062)mmol/L,(3.941±0.050)mmol/L and(3.158±0.041)mmol/L,respectively;for total glycerol(2.212±0.043)mmol/L,(1.679±0.033)mmol/L,(1.275±0.027)mmol/L and(1.067±0.023)mmol/L;for free glycerol(0.142±0.005)mmol/L,(0.149±0.004)mmol/L,(0.146±0.003)mmol/L and(0.122±0.003)mmol/L;and for triglycerides(2.069±0.043)mmol/L,(1.530±0.033)mmol/L,(1.129±0.027)mmol/L and(0.945±0.023)mmol/L.Conclusion Certified reference materials for serum total cholesterol,total glycerol,free glycerol and triglycerides have been prepared.
