The effects of nitric oxide on the cardiac function of ischemic and reperfused myocardium of rats in vivo
- VernacularTitle:L-精氨酸——一氧化氮代谢通路对在体心肌缺血/再灌注大鼠心功能的影响
- Author:
Feng LIANG
;
Xiaoliang WANG
;
Wenliang MA
;
Yu TIAN
;
Jin WANG
;
Xiaoyu LI
;
Yiyong HAO
;
Huirong LIU
- Publication Type:Journal Article
- Keywords:
Nitric oxide;
Myocardial reperfusion injury;
Apoptosis;
Cardial functions
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2008;24(2):115-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether nitric oxide may result in different effects on cardiac function at the different time points during the course of myocardial ischhemia and reperfusien of rats in viwo, and if so, to investigate the mechanisms involved.Methods Male adult rats were subjected to 30 minutes of myocardial ischemia followed by 5 hours of reperfusien. L-arginine was administrated at a bolus at both 10 minutes before and 50 minutes after reperfusion in early treatment group. The L-arginine was administrated at a bolus at both 3 and 4 hours after reperfusion in late treatment group. The following variables were measured: cardiac hemodynamic parameters, myocardial easpase 3 activity, iNOS and total NO content. Results Compared with vehicle group, administration of L-arginine at early reperfusion markedly reduced myocardial caspase 3 activity, and improved cardiac function. In strict contrast, administration of L-arginine at late reperfusion resulted in a significant increase in myocardial NOx content, myocardial apoptosis, and depressed the cadiac function. Conclusion Timely administration of L-arginine might inhibit apoptosis while late administration of L-arginine might enhance apoptosis.
