Role of heparanase in the pathogenesis of proteinuria in diabetic nephropathy rats
- VernacularTitle:乙酰肝素酶在糖尿病肾病大鼠蛋白尿发生中的作用
- Author:
Lin TANG
;
Yuhua WANG
;
Zhangsuo LIU
;
Jing XIAO
;
Yanna DOU
;
Ji DONG
- Publication Type:Journal Article
- Keywords:
Heparanase;
Diabetic nephropathy;
Proteinuria
- From:
Chinese Journal of Nephrology
2008;24(4):277-281
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of heparanase(HPA)in kidney of diabetic nephropathy(DN)rats and to investigate the role of HPA in the pathogenesis of proteinuria in DN rals. Methods DM rat models induced by intraperitoneal injection with streptozotocin were constnmted.Twenty-six rats were randomly divided into heahhy control group(n=6),DM6-week group(n=10)and DM 12-week group(n=10).Relative kidney weight(RKW),blood glucose,BUN,Scr,24-hour urine volume and(t 24-hour proteinuria quantilation were measured,and renal morphology was observed after 6 and 12 weeks.The expression of HPA was examined by immunohistochemistry and reverse transcription PCR. Results (1)Conq)ared with the control group,RKW,blood glucose,BUN,24-hour urine volume and 24-hour proteinuria quantitation of DM groups increased markedly(P＜0.05 orP＜0.01).(2)Compared to the control group,the expression of HPA mRNA and protein in DM groups increused significantly(P＜0.01).(3)HPA protein and mRNA were positively correlated with the quantification of urinary prolein (r=0.783,P＜0.01;r=0.793,P＜0.01). Conclusion The increased expression of HPA maybe parlicipate in the pathogenesis of proteinuria in DN.
