Isolation and identification of mixed erythrocytes with different blood groups from the patients following ABO-incompatible allogeneic haematopoietic stem cell transplantation
- VernacularTitle:ABO血型不合异基因造血干细胞移植后不同血型混合红细胞的分离鉴定
- Author:
Jianping YIN
;
Yicheng ZHANG
;
Wenqiang WANG
;
Xiaoling SHI
;
Wei HUANG
;
Yi XIAO
- Publication Type:Journal Article
- Keywords:
ABO blood-group system;
Blood group incompatibility;
Hematopoietic stem cell transplantation;
Erythrocytes;
Cell separation
- From:
Chinese Journal of Laboratory Medicine
2008;31(2):159-162
- CountryChina
- Language:Chinese
-
Abstract:
objective To establish a new method for isolation and identification of mixed erythrocytes with different blood groups from the patients following ABO-incompatible allogeneic haematopoietic stem cell transplantation(allo-HSCT),and investigate its application in post-transplantation survival erythrocytes.Methods The erythrocyte blood group antigens from the patiehts following ABO-incompatible allo-HSCT were agglutinated by the antibodies known.centrifuged at 800×g and 50×g to isolate agglutinated and unagglutinated erythrocytes respectively step by step.These erythrocytes collected were counted and identification respectively.Results The sensitivity,accuracy,recovery and reproducibility of the new method were 1%,100%,92.5%and 100%,respectively.The post-transplantation survival erythrocytes mixed with patient's erythrocytes from 18 patients following ABO-incompatible allo-HSCT were successfully isolated and identified by provided methods.It was the first time to identify the survival erythrocytes 11 th to 72 th day after allo-HSCT.The amount of the survival erythrocytes had been changing following post-transplantation periods.Conclusions The method can be widely employed in the isolation and identification of mixed erythrocytes with different blood groups.It provides reliable method to study the clinic significance of the changes of the survival erythroeytes from the patients following ABO-incompatible allo-HSCT.
