Detection and identification of human metapneumovirus infection in Shenzhen children
- VernacularTitle:深圳地区儿童呼吸道感染人类偏肺病毒检出及鉴定
- Author:
Xuedong LU
;
Changdong LU
;
Yuejie ZHENG
;
Qiong WANG
;
Yinhui ZHANG
;
Laizhi YANG
;
Jian LIU
- Publication Type:Journal Article
- Keywords:
Respiratory tract infections;
Metapneumovirus;
Nucleocapsid proteins;
Sequenceanalysis,DNA;
Polymerase chain reaction;
Child
- From:
Chinese Journal of Laboratory Medicine
2008;31(5):533-535
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect human metapneumovirus (hMPV)in respiratory intection rapidly and perform molecular analysis of hMPV.Methods Seven respiratory tract virus(11 subtypes)were assessed using multiplex PCR technology and flexible Multi-Analyte Profiling(suspension array).Human metapneumovirus was confirmed by using a real.Time reverse ranscriptase CR(RT-PCR)assay followed by sequencing.The cladogram analysis was performed further.Results The virus were detected in 40.2%(19/47)samples collected from clinicsl respiratory tract infections,including 8(42.1%)HRSV,7(36.8%)influenza virus,1(5.3%)parainfluenza virus,1(5.3%)rhinovirus,1(5.3%) coxsackievirus and 1(5.3%)human etapneumovirus infections.This is the first time that hMPV was deteced from clinical samples in Shenzhen.The sequencing of specific fragment of neucleoprotein of hMPV showed this hMPV shares over 98% homology with Beijing strain.Japan strain and Thailand strain.The cladogram analysis showed that they were in the same cluste.Conclusions Human etapneumovirus is a maior cause of children respiratory tract disease. Multiplex PCR technology and nexible Multi-Analyte Profiling were hish sensitive and high-throughput for detection of human metapneumovirus.They axe very robust and applicable in etiology analysis.
