The establishment of immunochromatographic tests trips with colloidal gold for early and rapid diagnostic of hemorrhagic fever with renal syndrome
- VernacularTitle:早期快速诊断肾综合征出血热胶体金试纸条的研制
- Author:
Lijuan XIONG
;
Fengju SU
;
Linglan ZENG
;
Lihua HU
- Publication Type:Journal Article
- Keywords:
Hemorrhagic fever with renal syndrome;
Gold colloid;
Reagent strips;
Immunoglobulin M;
Nucleocapsid proteins
- From:
Chinese Journal of Laboratory Medicine
2008;31(6):699-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an early,simple and rapid colloidal gold strip method for the detection of IgM against hantavirus nucleocapsid protein in patients with hemorrhagic fever with renal syndrome(HFRS).Methods Purified recombinant Hantavirus nucleoprotein(rNP)was labeled by colloidal gold particles and then sprayed and fixed on fiberglass membrane as the combination pad.Anti-Human IgM(μ-chain specific)antibody produced in goat was fixed in the detection area,and mouse antihantavirus antibody was fixed in the quality control area.Both of them were on nitrocellous membrane strip in tandem.Together with a specimen pad ahead.The conbination pad and the nitrocellous membrane were assembled into a test strip.The colloidal gold strip assay was compared with ELISA for evaluation of specificity and sensitivity.Results The colloidal gold strip tests showed positive in the serum samples from 50 cases of HFRS which was clinically diagnosed and then verified by ELISA within 10-15 minutes.Whereas 30 serum samples of healthy donors have tested negative.Conclusions Our new colloidal gold immunochromatographic test strip method was well concordant with the ELISA assay,but the former was more raoid and simole.It could be used primary medical services.
