The effect of pigment epithelium-derived factor on apoptosis of glioma cells
- VernacularTitle:色素上皮细胞衍生因子对脑胶质瘤细胞凋亡的影响
- Author:
Tao ZHANG
;
Ming GUAN
;
Yuan Lü
- Publication Type:Journal Article
- Keywords:
Glioma;
Eye proteins;
Herve growth factor;
Serpins;
Protein p16;
Apoptosis
- From:
Chinese Journal of Laboratory Medicine
2008;31(4):421-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the impact of pigment epithelium-derived factor(PEDF)on the proliferation and apoptosis of the glioma cells by detecting expression of apoptosis related proteins.Methods U87 cells were treated with PEDF(1000μg/ml,U87PEDF),or without PEDF(U87com0),cell proliferation assays were performed by MTT assay to test the effect of PEDF on proliferation of glioma cells;Apoptosis assays were performed by flow-cytometric analysis;Western-blot Was used for evaluating the expression of p16 protein.Results The induced inhibitony rates of glioma cells by PEDF were(54.29±0.62)% Compaxed with the control(t=2.63,P<0.05).The apoptosis assay showed that(21.84±0.36)% of PI- negative/annexin V-positive Was present in the U87 PEDF cells.The appoptosis was associated with the incteases of p16 protein(0.82±0.09)compared with tlle control(0.43±0.03,P<0.05).Conciusion PEDF may play a significant role in apoptosis regulation and proliferation of glioma cell accompanied with the increase of the p16 protein.
