Study on hepatocyte cell infected in vitro by hepatitis C virus positive serum
- VernacularTitle:丙型肝炎病毒阳性血清体外感染人肝细胞的研究
- Author:
Yan LIU
;
Zibai QI
;
Heqiu ZHANG
;
Hemin LI
;
Jinlian GU
;
Yang YU
;
Zhen YANG
;
Kun CHEN
- Publication Type:Journal Article
- Keywords:
Hepatitis C virus;
Human hepatocyte;
Infection;
Replication
- From:
Chinese Journal of Microbiology and Immunology
2008;28(5):440-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the hepatocyte cells infected by hepatitis C virus (HCV) positive serum. Methods Human hepatocyte 7701 was incubated with HCV RNA-positive and HCV antibody(Ab) negative sera BP52. Then, the expression of HCV antigen and the presence of HCV-RNA in cell and supernatant were assayed by RT-PCR, sequence analysis, immunofluorescent staining, Western blot, confocal laser microscopy. The ultrastructural changes of infected cells were observed by electro-microscopy. Results Plus-strand RNA and minus-strand RNA were intermittently detected in cell and/or supernatant on day 7-45 after infection. Sequence analysis demonstrated that the positive DNA nucleic acids were identified with HCV 5′-non-coding region(NCR) sequence. HCV core and NS3 protein were expressed in cytoplasm of infected cells. After 2 or 3 weeks, obvious intracellular ultrastructural changes and virus-like particles were observed. Conclusion human hepatocyte 7701 could support replication of HCV in vitro, which could be a useful tool for setting up cell model of HCV infection and studying the mechanism of HCV infection.
