Effect of fastigial nucleus stimulation on the expression of matrix metalloproteinase-9 after cerebral ischemia
- VernacularTitle:小脑顶核电刺激对大鼠脑缺血后基质金属蛋白酶-9表达的影响
- Author:
Jian WANG
;
Weiwei DONG
;
Zhanlong ZHANG
- Publication Type:Journal Article
- Keywords:
Fastigial nucleus;
Cerebral ischemia;
Blood-brain barrier;
Matrix metalloproteinase-9
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2008;30(5):294-297
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of preconditioning through stimulation of the fastigial nucleus (FNS)on the expression of matrix metalloproteinase-9 after cerebral ischemia. Methods A total of 312 male Wistar rats were divided randomly into three groups:a control group,a sham-FNS group and a FNS group.All the rats in the sham-FNS and FNS groups were subjected to middle cerebral artery occlusion(MCAO)by using a thread to occlude the right middle cerebral artery,while those in the control group were subject only to a similar operative procedure without real MCAO.Twenty-four hours before the operation,all the rats in the FNS group were given 1 hour of preconditioning FNS,while those in the sham-FNS group was given sham-FNS.Individuals were sacrificed at the 6th,12th,24th,and 72nd hour after MCAO.The brain water content was measured by the dry-wet weight method.The blood-brain barrier(BBB)permeability was measured using Evan's blue.MMP-9 protein expression was measured by Western blotting.Results The water content in the ischemic brain tissue,the BBB permeability and the expression of MMP-9 were all found to be significantly higher in the sham-FNS group than in the control group.Compared with the sham-FNS group,the brain water content and BBB permeability were significantly lower in the FNS group,while M MP-9 protein expression also decreased significantly.Conclusion FNS preconditioning can inhibit MMP-9 expression,thereby relieving BBB permeability and ischemic brain edema.
