Effects of electroacupuncture on the expression of caspase-3 in the ischemie penumbra in the early stage of cerebral ischemia
- VernacularTitle:电针对大鼠脑缺血后早期半暗带区Caspase-3表达的影响
- Author:
Baojuan CUI
;
Miao JIANG
;
Shanshan WANG
;
Qiangsan SUN
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Electroacupuncture;
Caspase-3
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2008;30(6):374-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of electroacupuncture on expression of caspase-3 in the is- chemic penumbra in the early stage of cerebral ischemia in rats. Methods 100 Wistar rats were randomly divid- ed into a sham-operated group, a model group and an electroacupuncture group. The middle cerebral artery was ex- perimentally occluded in the rats in the model and electroacupuncture groups, while a sham operation without cere- bral artery occlusion was carried out on the rats in the sham-operated group. Immunocytochemistry was employed to detect the expression of caspase-3 in the ischemic penumbra at 24 h, 48 h and 72 h after cerebral ischemia. Re- suits There was almost no positive expression of caspase-3 in the sham-operated rats. Numerous caspase 3-posi- tive cells were found in the ischemic penumbra in the model and electroacupuncture groups. The peak of expression was reached 24 hours after cerebral ischemia. The number of caspase 3-positive cells was significantly greater in the model group than in the electroacupuncture group at 24 h and 48 h. Conclusion The increase of caspase 3-posi- tive cells in the ischemic penumbra is time-dependent. Electroacupuncture can significantly decrease the expression of caspase-3.
